Library preparation

Part of our library preparation service, we will offer advice and guide you in choosing the best approach/kit for your study. We very frequently work on niche projects and will use our technical knowledge to develop a custom solution for you. Do not hesitate to discuss with us your project, we can design your experiment and tailor it to your objectives.

We will check the quality of samples and advise you when necessary should the samples not meet the quality requirements. This could involve replacing samples, cleaning them up or suggest a more suitable solution if available. For more information go to Sample QC.

CGS has worked with numerous kits over the years, we have expertise in choosing the best kit for your project.

The type of library preparation that we are offering for both Illumina and Nanopore platforms.

• RNASeq: mRNA or total RNA, for low to high amount, usually strand specific library prep
• DNA and amplicon: library preparation for DNA sequencing and amplicon. Typical applications: Whole Genome Sequencing, Whole Genome Methylation, CRISPR panel, Amplicon panels.
• ATACSeq and ChIPSeq: preparation of pull down material for sequencing
• Custom and bespoke library preparation: we can offer custom and bespoke library preparation based on publish protocol as long as they are clearly detailed and optimised
• Panel and sequence capture: library preparation using sequence capture to target specific reagion of the genome, e.g. Exome sequencing, targeted methylome
• Metagenomic and microbiome, our facility have a great expertise with these type of assays. 

Library preparation more specific to the Illumina platform:

We have option for working with degraded and low amounts of DNA/RNA. If you have a specific library preparation method you would like to use, please do get in touch.