Fragment Analysis

Fragment analysis

3730XL DNA analyser

Instrument from ThermoFisher (formally Applied Bioystems), it is a 96-capillary array DNA sequencer for Fragment Analysis.

  • DNA fragments are sized by comparison with a standard size marker, loaded with each sample
  • Fluorescent labeling enables to analysis of many independent PCR products (loci) in the same capillary injection
  • Applications include Microsatellite analysis, SNP genotyping, loss of heterozygosity (LOH) analysis, Multiplex Ligation-Dependent Probe Amplification (MLPA), Methylation specific MLPA

The DNA Sequencing Facility offers Fragment Analysis runs on the ABI 3730XL, a 96-capillary instrument with a 50cm array.

3730xl DNA analyser

Fragment analysis applications are those in which fluorescent fragments of DNA (produced by PCR using primers specifically designed for an experiment) are separated using capillary electrophoresis and sized by comparison with a standard. 

Most fragment analysis applications have simplified workflows—straightforward sample preparation (without the purification required for sequencing samples), the ability to multiplex, and applications can all be separated using the same capillary electrophoresis polymer.

Fragment analysis overview

What can I do with fragment analysis?

• Microsatellite (STR) analysis 

• SNP Genotyping

 • Fingerprinting

 • Relative Fluorescence Quantitation

Sample requirements

Fragment Analysis plates are supplied by the user ready to load on the instrument, i.e. customers complete the PCR reactions using their specific fluorescent labelled dye/s and add the appropriate size standard.

Pricing

We offer Fragment Analysis runs for a price of £50 per plate for full 96-well plates.