Library Preparation - old

Part of our library preparation service, we will offer advice and guide you in choosing the best approach/kit for your study. We very frequently work on niche projects and will use our technical knowledge to develop a custom solution for you. Do not hesitate to discuss with us your project, we can design your experiment and tailor it to your objectives.

We will check the quality of samples and advise you when necessary should the samples not meet the quality requirements. This could involve replacing samples, cleaning them up or suggest a more suitable solution if available. For more information go to Sample QC.

CGS has worked with numerous kits over the years. Listed below are our preferred assays for the various possible applications. Please let us know if you would like to use a different kit not listed here.

RNA Solutions

• 3' messenger RNA - QuantSeq 3' mRNA (lexogen)
• messenger RNA - TruSeq Stranded mRNA (Illumina)
• Total RNA - TruSeq Stranded Total RNA (Illumina)
• Small RNA - NEXTflex small RNA-Seq (Bioo Scientific)
• Low input mRNA - SMART-Seq v4 Ultra Low input RNA (Clontech) + Nextera XT (Illumina)
• Low input total RNA - Ovation® Singe Cell RNA-Seq (Nugen)
• FFPE Total RNA - TruSeq RNA Access (Illumina)
• Total RNA for non standard species - Ovation® Universal RNA-Seq System (Nugen)

DNA Solutions

• Whole-Genome (covaris fragmentation) - Kapa Hyper Prep (KapaBiosystems)
• Whole-Genome (enzymatic fragmentation) - Kapa Hyper Plus (KapaBiosystems)
• Small Whole-Genome - Nextera XT (Illumina)
• Exome - Nextera Rapid Capture Exome or TruSeq Rapid Exome and TruSeq Exome (Illumina)
• CHIP-Seq - Kapa Hyper Prep (KapaBiosystems)
• Amplicon - Kapa Hyper Prep (KapaBiosystems)
• Methyl-Seq - Agilent SureSelect^XT Human Methyl-Seq (Agilent) or Ovation® RRBS Methyl-Seq System (Nugen)
• Metagenomics - NEXTflex™ 16S V4 Amplicon-Seq Kit 2.0 or NEXTflex™ 16S V1 - V3 Amplicon-Seq or NEXTflex™ 18S ITS Amplicon-Seq (Bioo Scientific)